Circadian and Circaseptan Dependence of the Beta-atp Peak of Four Different Cancer Cell Cultures: Implications for Chronoradiotherapy

K e y w o r d s Circadian and circaseptan rhythm, Beta-ATP peak, Cancer cell cultures, Implications for chronoradiotherapy INTRODUCTION Multifrequency rhythms, chaotic-appearing changes, and trends in their characteristics are time structures within and around us. Interplanetary, planetary, hypothalamic and pineal intermodulations complement pineal, pituitary and adrenal interactions linked to periodic DNA and thus to growth, normal and abnormal. Sunshine by the day and geomagnetics by night with a yearly and half yearly variation modulate circulating human melatonin (1). The present paper is aimed at studying the time-dependence of the beta-ATP peak of different tumor cells in vitro as a gauge of overall metabolism. MATERIALS AND METHODS Four different cell cultures were studied as tumor spheroids in the stationary phase: C3H-MA grown from a murine mammary adenocarcinoma; B14 cells from a Chinese hamster; 9L glioma cells of a rat, and a monolayer culture of mouse L1210 leukemia cells. All cell cultures were irradiated with 6 MeV electrons (VARIAN Clinac linear accelerator C/D 2300). The dishes containing the cell colonies and the nutrient medium were positioned between two foils in order to reach the maximal dose at the surface of the dishes; the second foil served as back scatter medium.